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DNA gel electrophoresis: Effect of field intensity and agarose concentration on band inversion
Author(s) -
Slater Gary W.,
Turmel Chantal,
Lalande Marc,
Noolandi Jaan
Publication year - 1989
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360281012
Subject(s) - reptation , chemistry , agarose , electrophoresis , electric field , gel electrophoresis of nucleic acids , agarose gel electrophoresis , dna , gel electrophoresis , inversion (geology) , analytical chemistry (journal) , chemical physics , chromatography , polymer , biochemistry , physics , paleontology , organic chemistry , quantum mechanics , structural basin , biology
We study the effect of electric field intensity and agarose gel concentration on the anomalous electrophoretic mobility recently predicted by the biased reptation model and experimentally observed for linear DNA fragments electrophoresed in continuous electric fields. We show that high fields and low agarose concentrations eliminate the physical mechanism responsible for anomalous DNA mobility and band inversion, in good agreement with theory, thus restoring the monotonic mobility–size relationship necessary for unambiguous interpretation of the results of DNA gel electrophoresis.