Premium
Solvated helical backbones: X‐ray diffraction study of Boc‐Ala‐Leu‐Aib‐Ala‐Leu‐Aib‐OMe · H 2 O
Author(s) -
Karle I. L.,
FlippenAnderson J. L.,
Uma K.,
Balaram P.
Publication year - 1989
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360280307
Subject(s) - chemistry , hydrogen bond , molecule , crystallography , peptide , stereochemistry , ether , helix (gastropod) , organic chemistry , snail , biology , ecology , biochemistry
A second example of insertion of a water molecule into the helical backbone of an apolar peptide is presented here and compared to a similar occurrence in a longer peptide with the same type of sequence of residues, i.e., Boc‐Aib‐(Ala‐Leu‐Aib) 3 ‐OMe. The backbone of the title compound assumes an approximate 3 10 ‐helical form with three 4 → 1 hydrogen bonds. In the place of a fourth 4 → 1 hydrogen bond, a water molecule is inserted between O(1) and N(4), and acts as a bridge by forming hydrogen bonds N(4) … W(1) (2.95 Å) and W(1) … O(1) (2.81 Å). The water molecule participates in a third hydrogen bond with a neighboring peptide molecule, W(1) … O(4) (2.91 Å). The insertion of the water molecule causes the apolar peptide to mimic an amphiphilic helix. Crystals grown from ethyl acetate/petroleum ether (reported here) or from methanol/water solution are in space group P2 1 2 1 2 1 with a = 12.024(4) Å, b = 15.714(6) Å, c = 21.411(7) Å, Z = 4 and d calc = 1.124 g/cm 3 for C 32 H 58 N 6 O 9 · H 2 O. The overall agreement factor R is 6.3% for 2707 reflections observed with intensities > 3σ( F ) and the resolution is 0.90 Å.