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Synthesis of the dodecapeptide corresponding to domain III of bovine brain calmodulin: α‐β Shift side reactions during the synthesis by the classical method in solution
Author(s) -
Borin Gianfranco,
Calderan Andrea,
Ruzza Paolo,
Pezzoli Anna,
Marchiori Fernando,
Peggion Evaristo
Publication year - 1989
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360280133
Subject(s) - calmodulin , chemistry , sequence (biology) , fragment (logic) , condensation reaction , calcium , stereochemistry , combinatorial chemistry , biophysics , biochemistry , algorithm , organic chemistry , catalysis , computer science , biology
We report details of the chemical synthesis of the dodecapeptide corresponding to the calcium binding loop III of bovine brain calmodulin (sequence 93–104) and its fragments 96–04, 93–98, and 99–104. The preparation of the peptides employed classical solution methods and a fragment‐condensation strategy. The major difficulties were encountered during the synthesis of the peptides containing the N‐terminal sequences‐Gly‐Asn‐Gly‐ and ‐Asp‐Lys‐Asp‐Gly‐Ans‐Gly‐, in which α‐β shift side reactions were observed.