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Cluster analysis of protein fourier transform infrared spectra
Author(s) -
Lipkus Alan H.,
Lenk Thomas J.,
Chittur Krishnan K.,
Gendreau R. Michael
Publication year - 1988
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360271111
Subject(s) - chemistry , cluster (spacecraft) , infrared spectroscopy , spectral line , analytical chemistry (journal) , bovine serum albumin , fourier transform , fourier transform infrared spectroscopy , infrared , adsorption , chromatography , organic chemistry , optics , physics , quantum mechanics , astronomy , computer science , programming language
Cluster analysis techniques were used to examine a set of Fourier transform infrared (FT‐IR) spectra of bovine serum albumin (BSA) in the adsorbed and nonadsorbed states. The region from 1480 to 1600 cm −1 , comprising the amide II band, was used. Spectra were preprocessed to compensate for linear baseline variation, and the single linkage method of cluster analysis was applied. As expected, the spectra of adsorbed and nonadsorbed BSA fell into two distinct clusters. However, no further clustering was observed among the adsorbed BSA spectra on the basis of surface type, suggesting that surface specificity of the spectral changes induced in BSA by adsorption is not detectable above experimental variation. This work illustrates the value of using cluster analysis in the FT‐IR study of proteins as a complement to other data analysis methods.