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Electron microscopy of beaded agarose gels
Author(s) -
Attwood T. K.,
Nelmes B. J.,
Sellen D. B.
Publication year - 1988
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360270204
Subject(s) - agarose , electron microscope , chemistry , range (aeronautics) , fiber , microscopy , distribution (mathematics) , molecular physics , crystallography , optics , composite material , materials science , chromatography , physics , mathematics , mathematical analysis , organic chemistry
Electron microscopy has been carried out on sections of beaded agarose with a wide range of thicknesses, and the results have been analyzed by means of stereological theory using computer graphics. The results agree with a randomly orientated system in which, for 4% gels, the mean molecular weight per unit length of the fiber system is 110 kg mol −1 nm −1 , and the number average interjunction length is 37 nm, with an asymmetrical distribution resembling a Maxwell‐Boltzmann distribution. The spatial distribution of the structure is not uniformly random and there seem to be mi microvoids.