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A simplified analysis of scatchard plots for systems with two interacting binding sites
Author(s) -
Galley W. C.,
Bouvier M.,
Clas S.D.,
Brown G. R.,
StPierre L. E.
Publication year - 1988
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360270106
Subject(s) - scatchard plot , chemistry , stoichiometry , binding site , adsorption , molar ratio , polyacrylamide , ligand (biochemistry) , saturation (graph theory) , binding constant , chromatography , analytical chemistry (journal) , receptor , organic chemistry , biochemistry , polymer chemistry , mathematics , combinatorics , catalysis
A simple graphical method for calculating stoichiometric and site binding constants for systems with two initially equivalent interacting sites is derived from a modified Scatchard equation. The binding constants can be calculated from Scatchard plots ( r /[A] as a function of r ) using the values of r /[A] ( r is the molar ratio of bound ligands to total protein and [A] is the equilibrium concentration of free ligand) when r = 0 and r = 1 (half‐saturation). The applicability of the method to the adsorption of bilirubin by peptide pendants immobilized on a polyacrylamide support is demonstrated.