A simplified analysis of scatchard plots for systems with two interacting binding sites | Zendy

Galley W. C. | Zendy; Bouvier M. | Zendy; Clas S.D. | Zendy; Brown G. R. | Zendy; StPierre L. E. | Zendy

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A simplified analysis of scatchard plots for systems with two interacting binding sites

Author(s) -

Galley W. C.,

Bouvier M.,

Clas S.D.,

Brown G. R.,

StPierre L. E.

Publication year - 1988

Publication title -

biopolymers

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.556

H-Index - 125

eISSN - 1097-0282

pISSN - 0006-3525

DOI - 10.1002/bip.360270106

Subject(s) - scatchard plot , chemistry , stoichiometry , binding site , adsorption , molar ratio , polyacrylamide , ligand (biochemistry) , saturation (graph theory) , binding constant , chromatography , analytical chemistry (journal) , receptor , organic chemistry , biochemistry , polymer chemistry , mathematics , combinatorics , catalysis

A simple graphical method for calculating stoichiometric and site binding constants for systems with two initially equivalent interacting sites is derived from a modified Scatchard equation. The binding constants can be calculated from Scatchard plots ( r /[A] as a function of r ) using the values of r /[A] ( r is the molar ratio of bound ligands to total protein and [A] is the equilibrium concentration of free ligand) when r = 0 and r = 1 (half‐saturation). The applicability of the method to the adsorption of bilirubin by peptide pendants immobilized on a polyacrylamide support is demonstrated.

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