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Two‐dimensional 1 H‐nmr investigation of the water conformation of the atrial natriuretic factor (ANF 101–126)
Author(s) -
Theriault Y.,
Boulanger Y.,
Weber P. L.,
Reid B. R.
Publication year - 1987
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360260707
Subject(s) - chemistry , two dimensional nuclear magnetic resonance spectroscopy , nuclear magnetic resonance spectroscopy , spectroscopy , nuclear overhauser effect , spectral line , proton nmr , nuclear magnetic resonance , nmr spectra database , analytical chemistry (journal) , crystallography , stereochemistry , chromatography , physics , quantum mechanics , astronomy
The complete sequential assignment of the 1 H‐nmr resonance frequencies of the active fragment of the rat atrial natriuretic factor (ANF 101–126) has been performed. Two‐dimensional nmr techniques have been employed, including phase‐sensitive nuclear Overhauser spectroscopy (NOESY), relayed coherence transfer spectroscopy (RELAY), and J ‐correlated spectroscopy (COSY). Experiments were performed both in D 2 0 and H 2 0 solutions at different pH values. With few exceptions, resonance frequencies were practically pH independent. NOESY spectra were recorded using both 300‐ and 500‐ms mixing times, and no long‐range connectivities were observed, leading to the conclusion that ANF 101–126 has no defined secondary nor tertiary structure in water in the pH range used (2.73–5.21).