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Novel anchorage matrices for suspension culture of mammalian cells
Author(s) -
Bohak Z.,
Kadouri A.,
Sussman M. V.,
Feldman A. F.
Publication year - 1987
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360260018
Subject(s) - microcarrier , petri dish , laboratory flask , chemistry , dextran , cell culture , fibroblast , plasminogen activator , suspension culture , matrix (chemical analysis) , microbiology and biotechnology , cell , chromatography , biochemistry , biology , in vitro , genetics , endocrinology
Nonwoven fabrics were used as a support matrix for culturing anchorage‐dependent diploid human lung fibroblast (IMR‐90) cells. The most significant advantage of the fabrics is that low‐inocula concentrations suffice to attain high final cell density. Cultures were successfully grown from inocula containing as little as 5% of the final number of cells, which is significantly lower than the 30–40% inocula concentrations typically required for tissue cell culture on dextran bead microcarriers, or on petri dishes or culture flasks. Nonwoven fabric cell supports also were superior to conventional spherical microcarriers for production of metabolic biopolymers (t‐plasminogen activator) in serum‐free media.