Synthesis of fragments by classical solution methods for use in cytochrome c semisynthesis

We report details of the chemical synthesis of two fragments reproducing the C‐terminal sequences 71‐108 and 70‐108 of Saccharomices cerevisiae cytochrome c . Preparation of the fragments employed classical solution methods and a fragment‐condensation strategy; they have been used, together with a third fragment (sequence 67‐108) [L. Moroder, B. Filippi, G. Borin & F. Marchiori (1975) Biopolymers 14 , 2061–2074], in the semisynthesis of chimeric cytochromes [C. J. A. Wallace, G. Corradin, F. Marchiori & G. Borin (1986) Biopolymers 25 , 2121–2132].