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Synthesis of fragments by classical solution methods for use in cytochrome c semisynthesis
Author(s) -
Borin Gianfranco,
Marchiori Fernando,
Calderan Andrea,
Corradin Giampietro,
Wallace Carmichael J. A.
Publication year - 1986
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360251205
Subject(s) - semisynthesis , chemistry , cytochrome c , fragment (logic) , stereochemistry , sequence (biology) , template , combinatorial chemistry , biochemistry , nanotechnology , mitochondrion , materials science , computer science , programming language
We report details of the chemical synthesis of two fragments reproducing the C‐terminal sequences 71‐108 and 70‐108 of Saccharomices cerevisiae cytochrome c . Preparation of the fragments employed classical solution methods and a fragment‐condensation strategy; they have been used, together with a third fragment (sequence 67‐108) [L. Moroder, B. Filippi, G. Borin & F. Marchiori (1975) Biopolymers 14 , 2061–2074], in the semisynthesis of chimeric cytochromes [C. J. A. Wallace, G. Corradin, F. Marchiori & G. Borin (1986) Biopolymers 25 , 2121–2132].