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Molecular structure of the cis‐syn photodimer of d(TpT) (cyanoethyl ester)
Author(s) -
Hruska F. E.,
Voituriez L.,
Grand A.,
Cadet J.
Publication year - 1986
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360250804
Subject(s) - chemistry , orthorhombic crystal system , dihedral angle , cyclobutane , crystallography , stereochemistry , crystal structure , pyrimidine , ring (chemistry) , molecule , cyclohexane conformation , hydrogen bond , organic chemistry
The three‐dimensional structure was determined by x‐ray crystallography for d(T[p](CE)T), a uv photoproduct of the cyanoethyl (CE) derivative of d(TpT), having the cis‐syn cyclobutane (CB) geometry and the S‐configuration at the chiral phosphorus atom. The crystals of C 23 H 30 N 5 O 12 P · 2H 2 O belong to the orthorhombic space group P2 1 2 1 2 1 (Z = 4), with cell dimensions a = 11.596 Å, b = 14.834 Å, and c = 15.946 Å, containing two water molecules per asymmetric unit. The CB ring is puckered with a dihedral angle of 151°. The two pyrimidine bases are rotated by –29° from the position of direct overlap of their corresponding atoms. This represents a major distortion of DNA, since in DNA adjacent thymines are rotated by +36°. The pyrimidine rings are puckered with Cremer–Pople parameters for T[p] and in parentheses [p]T: Q : 0.24 Å (0.31 Å); θ: 123° (120°); ϕ: 141° (86°). These represent half‐chairs designated as 6 H 1 (T[p]) and 6 H 5 ([p]T). The CB and pyrimidine ring conformations are interrelated, and we postulate that they execute a coupled interconversion in solution. The T[p] segment has the syn glycosyl conformation, a 2 T 3 sugar pucker, and gauche − conformation at C4′‐C5′; the [p]T segment is anti , 3 T 4 , trans . The C5′‐O5′ torsion of the [p]T unit is –124.5°, and the C3′‐O3′ torsion of the T[p] unit is –152.9°. Bond angles and bond lengths involving the phosphorus atom are similar to those of other phosphotriesters. The P‐O3′ and P‐05′ torsion angles are –138.1° and 58.6°, respectively. Several intermolecular (but no intramolecular) hydrogen bonds are found in the crystal.