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Collagen fibrillogenesis in vitro : An investigation of the thermal memory effect and of the early events occurring during fibril assembly using dynamic light scattering
Author(s) -
Payne K. J.,
King T. A.,
Holmes D. F.
Publication year - 1986
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360250703
Subject(s) - fibrillogenesis , fibril , chemistry , dynamic light scattering , biophysics , monomer , in vitro , scattering , thermal , molecule , phase (matter) , chemical physics , crystallography , biochemistry , optics , materials science , nanotechnology , thermodynamics , organic chemistry , physics , nanoparticle , biology , polymer
Dynamic light scattering has been used to characterize a variety of lathyritic rat skin collagen solutions. The technique was used to monitor the onset of fibril assembly in vitro and to investigate the thermal memory effect. Although the incorporation of thermal memory was demonstrated by reheating the sample and subsequently observing a shortened turbidimetric lag phase, no significant differences between naive solutions and ones exhibiting thermal memory could be detected using photon correlation spectroscopy. This suggests that subtle changes in the state of the collagen molecules rather than extensive changes in the degree of aggregation are responsible for the thermal memory effect. During fibrillogenesis, no large‐scale changes in the distribution of monomers or aggregates occur until near the end of the lag phase.