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Dependence of oxygen quenching of intercalated methylene blue triplet lifetime on DNA base‐pair composition
Author(s) -
Berkoff Bruce,
Hogan Mike,
Legrange Jane,
Austin Robert
Publication year - 1986
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360250211
Subject(s) - chemistry , methylene blue , quenching (fluorescence) , oxygen , oligonucleotide , methylene , molecule , base pair , photochemistry , composition (language) , helix (gastropod) , triplet state , dna , base (topology) , fluorescence , organic chemistry , biochemistry , catalysis , ecology , mathematical analysis , photocatalysis , quantum mechanics , snail , biology , physics , linguistics , philosophy , mathematics
Abstract We utilize the dynamic quenching of the triplet state of methylene blue by molecular oxygen to observe changes in the rate at which oxygen can penetrate the helix as a function of base‐pair composition. The results indicate that the interior of the oligonucleotide dA‐dT is more accessible than dG‐dC to small molecules such as dioxygen.