Sequence‐specific assignment of the backbone  1 H‐ and  31 P‐nmr lines in a short DNA duplex with homo‐ and heteronuclear correlated spectroscopy | Zendy

Frey M. H. | Zendy; Leupin W. | Zendy; Sørensen O. W. | Zendy; Denny W. A. | Zendy; Ernst R. R. | Zendy; Wüthrich K. | Zendy

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Sequence‐specific assignment of the backbone 1 H‐ and 31 P‐nmr lines in a short DNA duplex with homo‐ and heteronuclear correlated spectroscopy

Author(s) -

Frey M. H.,

Leupin W.,

Sørensen O. W.,

Denny W. A.,

Ernst R. R.,

Wüthrich K.

Publication year - 1985

Publication title -

biopolymers

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.556

H-Index - 125

eISSN - 1097-0282

pISSN - 0006-3525

DOI - 10.1002/bip.360241214

Subject(s) - heteronuclear molecule , chemistry , spectroscopy , nuclear magnetic resonance spectroscopy , two dimensional nuclear magnetic resonance spectroscopy , pulse sequence , duplex (building) , crystallography , sequence (biology) , dna , nuclear magnetic resonance , resonance (particle physics) , stereochemistry , physics , atomic physics , quantum mechanics , biochemistry

We report the assignment of the backbone 1 H‐ and 31 P‐nmr lines in the synthetic hexadeoxyribonucleotide pentaphosphate duplex d(GCATGC) 2 , using double quantum filtered 1 H‐ 1 H correlation spectroscopy, 1 H observed 1 H‐ 31 P heteronuclear correlation spectroscopy, and 31 P relayed 1 H‐ 1 H correlation spectroscopy. The strategy used enables one to make sequence‐specific resonance assignments without reference to a known or assumed conformation of the DNA fragment.

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