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Sequence‐specific assignment of the backbone 1 H‐ and 31 P‐nmr lines in a short DNA duplex with homo‐ and heteronuclear correlated spectroscopy
Author(s) -
Frey M. H.,
Leupin W.,
Sørensen O. W.,
Denny W. A.,
Ernst R. R.,
Wüthrich K.
Publication year - 1985
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360241214
Subject(s) - heteronuclear molecule , chemistry , spectroscopy , nuclear magnetic resonance spectroscopy , two dimensional nuclear magnetic resonance spectroscopy , pulse sequence , duplex (building) , crystallography , sequence (biology) , dna , nuclear magnetic resonance , resonance (particle physics) , stereochemistry , physics , atomic physics , quantum mechanics , biochemistry
We report the assignment of the backbone 1 H‐ and 31 P‐nmr lines in the synthetic hexadeoxyribonucleotide pentaphosphate duplex d(GCATGC) 2 , using double quantum filtered 1 H‐ 1 H correlation spectroscopy, 1 H observed 1 H‐ 31 P heteronuclear correlation spectroscopy, and 31 P relayed 1 H‐ 1 H correlation spectroscopy. The strategy used enables one to make sequence‐specific resonance assignments without reference to a known or assumed conformation of the DNA fragment.