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Light‐scattering study on chick‐embryo cartilage proteoglycan type H (PGH)
Author(s) -
Maeda Hiroshi,
Oike Yasuteru
Publication year - 1985
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360240305
Subject(s) - chemistry , proteoglycan , guanidine , cartilage , embryo , sodium , hydrochloride , mole , chromatography , biochemistry , anatomy , extracellular matrix , microbiology and biotechnology , organic chemistry , biology , medicine
Light‐scattering measurements were carried out on chick‐embryo cartilage proteoglycan type H (PG‐H) in three different media. The number of uronates per PG‐H was obtained as a measure of the molecular weight: 2.3 × 10 3 in 4 M GdnHCl, 7.1 × 10 3 in 0.3 M NaCl, and 5.7 × 10 3 in 0.04 g/dL SDS + 0.1 M NaCl (GdnHCl, guanidine hydrochloride; SDS, sodium dodecylsulfate). Self‐association of PG‐H occurred in 0.3 M NaCl and 0.04 g/dL SDS + 0.1 M NaCl.
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