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Diffusion of bovine serum albumin in a neutral polymer solution
Author(s) -
Phillies George D. J.
Publication year - 1985
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360240206
Subject(s) - chemistry , bovine serum albumin , polymer , diffusion , viscosity , albumin , serum albumin , chromatography , light scattering , analytical chemistry (journal) , scattering , thermodynamics , organic chemistry , biochemistry , physics , optics
The diffusion coefficient D of bovine serum albumin through various solutions (pH 7.0, 0.5 M NaCl) of polythylene oxide ( M w ∼ 1 × 10 5 , 3 × 10 5 ) was studied with quasielastic light scattering. In solutions of the 1 × 10 5 polymer solution at polymer concentrations above 0.5 g/L, D is considerably greater than would have been expected from the viscosity of water:polymer mixtures, the deviations being larger at low protein concentration that at high protein concentration. With either polymer, D falls with increasing protein concentration.