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Applications of fluorescence spectroscopy to molecular cytogenetics
Author(s) -
Latt Samuel A.,
Lalande Marc,
Kunkel Louis M.,
Schreck Rhona,
Tantravahi Umadevi
Publication year - 1985
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360240108
Subject(s) - chemistry , dna , fluorescence , metaphase , molecular cytogenetics , cytogenetics , chromosome , computational biology , microbiology and biotechnology , biophysics , biology , gene , biochemistry , physics , optics
Phenomena studied by fluorescence spectroscopy, including differential perturbations of dye fluorescence by variations in DNA composition and interdye energy transfer, can be applied to cytological studies of chromosome structure, replication, and repair. When combined with fluorescence‐activated flow sorting, fluorescent differentiation between metaphase chromosomes can be exploited to obtain recombinant DNA libraries enriched for all or parts of individual chromosomes. Cloned DNA segments from such libraries continue to find application in studies of human chromosome structure and function and in molecular linkage analyses, thus leading to molecular genetic investigations of human disease states.