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Calculation of the thermodynamic quantities of solvation for lysozyme and β‐lactoglobulin a in guanidinium chloride
Author(s) -
Lapanje Savo
Publication year - 1984
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360231010
Subject(s) - chemistry , lysozyme , solvation , enthalpy , gibbs free energy , thermodynamics , guanidinium chloride , entropy (arrow of time) , solvent , organic chemistry , enzyme , biochemistry , physics
The thermodynamic quantities of solvation, that is, Gibbs free energy, enthalpy, and entropy for the transfer of lysozyme and β‐lactoglobulin A from water to 6 M guanidinium chloride solution, were calculated from the respective contributions of constituent groups. Comparison of the calculated values with the experimental ones reveals satisfactory agreement for lysozyme, whereas for β‐lactoglobulin A, it is semiquantitative. Thus the method of calculation appears to be fundamentally sound. The reasons for the differences in calculated and experimental value are briefly discussed.