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Diffusion‐controlled protein–DNA association: Influence of segemental diffusion of the DNA
Author(s) -
Berg Otto G.
Publication year - 1984
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360231005
Subject(s) - chemistry , diffusion , dna , biophysics , thermodynamics , biochemistry , physics , biology
The intrachain reaction theory of Wilemki and Fixman [(1974) J. Chem. Phys. 60 , 866–877] is used to assess the influence of internal DNA motions on various protein–DNA association schemes. It is found that, for large proteins, the diffusional association rate can be totally dominated by these motions rather than by the free‐trans‐lational diffusion rates. Also, the time required for the diffusion together of two DNA segments is estimated. This estimate can be used to provide an upper limit for the rate of intrachain cyclization, and also for the effective intrachain transfer rate of a protein bound to a DNA chain.