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The effects of alkali‐metal chlorides and of spermidine and spermine on the swelling pressures of DNA gels in the ultracentrifuge
Author(s) -
Richard Alfred J.
Publication year - 1984
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360230714
Subject(s) - spermine , spermidine , chemistry , ultracentrifuge , alkali metal , dna , polyamine , swelling , chromatography , metal , biochemistry , polymer chemistry , organic chemistry , chemical engineering , enzyme , engineering
Abstract The effects is solution of the alkali‐metal chlorides on the gel‐like phase of DNA formed in the ultracentrifuge cell have been studied. The polycations, spermidine and spermine, also were shown to affect strongly the swelling pressure of the DNA gel, with evidence for the destabilization of DNA in very dilute spermine, below 10 −6 M , and for the collapse of DNA in both spermine and spermidine solutions above 10 −6 M .