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Conformation study on heparin by intermediate‐angle X‐ray scattering
Author(s) -
Yamaguchi Saburo,
Hayashi Hisao,
Hamada Fumiyuki,
Nakajima Akio
Publication year - 1984
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360230603
Subject(s) - chemistry , small angle x ray scattering , scattering , monte carlo method , heparin , crystallography , x ray , disaccharide , helix (gastropod) , molecular physics , stereochemistry , physics , optics , ecology , biochemistry , statistics , mathematics , snail , biology
The conformation of heparin in water was investigated by intermediate‐angle x‐ray scattering (IAXS). The theoretical scattering function for the coil conformation was calculated by the Monte Carlo method using the approximation of separable conformation energies and the conformation energies computed for two disaccharide pairs in heparin. From x‐ray scattering in a relatively small‐angle region, the conformation of heparin is not the ordered 2 1 helix conformation but the coil conformation obtained by the Monte Carlo calculation. It is expected, from x‐ray scattering in a relatively wide‐angel region, that the sulfate groups of heparin maintain about 7 Å between them.