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Interaction of poly(I)·poly(C) with trans ‐dichloro‐diammine‐platinum (II)
Author(s) -
Hermann Dominique,
Fazakerley G. Victor,
Houssier Claude,
Guschlbauer Wilhelm
Publication year - 1984
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360230510
Subject(s) - polynucleotide , chemistry , intramolecular force , platinum , cytidine , covalent bond , intermolecular force , stereochemistry , crystallography , catalysis , molecule , enzyme , organic chemistry , biochemistry
The covalent binding of trans ‐Pt (NH 3 ) 2 Cl 2 to the double‐stranded poly(I)·poly(C) follows three types of reactions, depending on r b and the concentration of polynucleotide in the reaction mixture. At r b ⩽ 0.1, the principal reaction is coordination to poly(I), giving rise to some destabilization of the double strand, as shown by uv and CD spectra, and a decrease in T m values, giving rise to free loops of poly(C). At higher r b and low polynucleotide concentration, the free cytidine bases react with platinum bound on the complementary strand to form intramolecular (interstrand) crosslinks that restabilize the double‐stranded structure. At high r b and high polynucleotide concentration, while the above reaction still occurs, the predominant one is the formation of intermolecular crosslinks. Under no conditions has strand separation been observed.