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Flexibility of collagen determined from dilute solution viscoelastic measurements
Author(s) -
Nestler F. Henry M.,
Hvidt Søren,
Ferry John D.,
Veis Arthur
Publication year - 1983
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360220710
Subject(s) - viscoelasticity , chemistry , relaxation (psychology) , viscosity , dynamic modulus , dilution , shear modulus , pronase , moduli , dynamic mechanical analysis , cylinder , thermodynamics , elastic modulus , analytical chemistry (journal) , chromatography , organic chemistry , physics , mechanical engineering , psychology , social psychology , trypsin , quantum mechanics , engineering , enzyme , polymer
The frequency dependences of the storage and loss shear moduli, G ′ and G ″, of pronase‐treated collagen dissolved in acetate buffer at pH 4.0 were measured at 17.0°C by use of the Birnboim‐Schrag multiple lumped resonator apparatus. Some of the solutions contained 70% glycerol. The infinite‐dilution moduli were determined and compared with theoretical models for a rigid cylinder and a semiflexible rod. Only the latter could fit the data. A rotational time of 144 μ s and a slowest flexural relaxation time of 21 μ s , both reduced to water at 20°C, were determined from the fit. The intrinsic viscosity and rotational relaxation time correspond to a semiflexible rod with persistence length of about 170 nm and a Young's modulus of 4 × 10 10 dyn/cm 2 .