Premium
Methods for the analysis of the kinetics of cooperative binding of ligands to a two‐site lattice
Author(s) -
Edgerton Mary E.,
Handler Frank A.,
Wu ChengWen
Publication year - 1983
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360220215
Subject(s) - chemistry , singular perturbation , kinetic energy , kinetics , lattice (music) , perturbation (astronomy) , statistical physics , computational chemistry , mathematical analysis , physics , mathematics , quantum mechanics , acoustics
Solutions are obtained that describe the time dependence of the reversible binding of a ligand to a two‐site lattice. The binding may be cooperative. Three methods are used to obtain these solutions: the separation of on/off processes with a variable transformation, the asymptotic series analysis, and the singular perturbation procedure. Applications to parameter calculation from experimental data are presented. This kinetic system is such that it is difficult to extract all kinetic parameters from data analysis, and the implications for each method are also discussed.