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Effect of ionic strength on the diffusion coefficient of chondroitin sulfate and heparin measured by quasielastic light scattering
Author(s) -
Tivant P.,
Turq P.,
Drifford M.,
Magdelenat H.,
Menez R.
Publication year - 1983
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360220209
Subject(s) - chemistry , diffusion , ionic strength , relaxation (psychology) , macromolecule , electrolyte , light scattering , scattering , analytical chemistry (journal) , thermodynamics , chromatography , aqueous solution , optics , electrode , psychology , social psychology , biochemistry , physics
The normal modes for a mixture of charged macromolecules and electrolyte solution are calculated. We derive a generalized Debye relaxation time and the apparent diffusion coefficient of the macroion, which is shown to increase from its Stokes value, obtained in excess of added salt, with decreasing ionic strength. We test our result with experimental data for macromolecules with different charge densities: heparin and chondroitin sulfate. Besides, we show for this latter molecule that while the diffusion coefficient is increased, the scattered intensity is decreased but not by the same factor. Our results are compared with other theories developed in quasielastic light scattering.