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Immunoassays of peptide hormones and their chemical aspects
Author(s) -
Moroder L.,
Nyfeler R.,
Gemeiner M.,
Kalbacher H.,
Wüunsch E.
Publication year - 1983
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360220161
Subject(s) - chemistry , gastrin , conjugate , peptide , immunoassay , context (archaeology) , combinatorial chemistry , substrate (aquarium) , molecule , derivative (finance) , peptide hormone , fluorescence , thiol , biochemistry , hormone , organic chemistry , antibody , secretion , mathematical analysis , paleontology , oceanography , physics , mathematics , quantum mechanics , economics , financial economics , immunology , biology , geology
The N‐terminal maleoyl‐β‐alanyl derivative of human gastrin‐[2–17] has been synthesized as a model compound to investigate the usefulness of such peptide derivatives for their mild and selective conjugation, via reaction with thiol groups, to high‐molecular‐weight carrier molecules to produce antigens, as well as to radioiodinable or fluorogenic molecules to prepare tracers for immunoassays. In this context the examined enzyme substrate‐gastrin conjugate was found to exhibit the full immunoreactivity of the parent peptide and to be well suited as tracer in a gastrin fluorescence‐immunoassay.