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NMR analysis of structure and function of snake neurotoxins
Author(s) -
Miyazawa Tatsuo,
Endo Toshiya,
Inagaki Fuyuhiko,
Hayashi Kyozo,
Tamiya Nobuo
Publication year - 1983
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360220122
Subject(s) - chemistry , naja , amide , neurotoxin , crystal structure , nmr spectra database , stereochemistry , crystallography , venom , spectral line , biochemistry , physics , astronomy
The 270‐MHz proton‐nmr spectra of short neurotoxins (erabutoxins from Laticauda semifasciata and cobrotoxin from Naja naja atra ) and long neurotoxins (toxin B from Naja naja and α‐bungarotoxin from Bungarus multicinctus ) have been analyzed. The conformation of erabutoxin b in solution is largely consistent with the x‐ray crystal analysis, although the environment of His‐7 in solution is definitely different from that in the crystal. The pH‐dependent transition has been found for toxin B, indicating that the conformation in neutral solution is different from that in the crystal as grown from acidic solution. The deuterium‐exchange rates of the amide protons for the four neurotoxins have been measured. The order of structural rigidity is the same as the order of the irreversibility of neuromuscular block by neurotoxins.