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A circular dichroism microspectrophotometer
Author(s) -
Maestre M. F.,
Katz J. E.
Publication year - 1982
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360210916
Subject(s) - circular dichroism , chemistry , microscope , dna , spectral line , analytical chemistry (journal) , crystallography , optics , chromatography , biochemistry , physics , astronomy
A microscope capable of measuring the CD of intact single eukaryotic cells, DNA microcrystals, and other microscopic structures has been constructed and tested. It can measure the CD spectra in the 200‐ and 800‐nm wavelength range and consists of a modification to a standard Cary 60 CD machine in combination with a Zeiss uv microspectrometer. Preliminary CD spectra of red blood cells and lymphocytes are presented.