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Ligand‐induced melting reaction of specific‐sequence DNA molecules
Author(s) -
Tachibana Hideki,
Wada Akiyoshi
Publication year - 1982
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360210914
Subject(s) - chemistry , ligand (biochemistry) , macromolecule , helix (gastropod) , molecule , dna , sequence (biology) , crystallography , block (permutation group theory) , organic chemistry , receptor , biochemistry , ecology , snail , biology , geometry , mathematics
An algorithm for the calculation of ligand‐induced helix–coil transitions of macromolecules of any specific sequence was derived. The probabilities of each residue to be in helix and ligand‐free, helix and ligand‐bound, coil and ligand‐free, and coil and ligand‐bound states can be calculated by extending the recursion relation formula proposed by D. Poland [(1974) Biopolymers 13 , 1859–1871]. Calculations using hypothetical DNAs having block heterogeneities for melting reactions showed that cooperative binding specific to single strands weakens the effect of the heterogeneity. Fine structures in the melting profiles or cooperatively melting regions, which have so far been found in thermal melting experiments, were predicted to exist in ligand‐induced melting reactions for natural DNA fragments.