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Theoretical study of the fraction of a long‐chain DNA that can be incorporated in a recombinant DNA partial‐digest library
Author(s) -
Seed Brian
Publication year - 1982
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360210909
Subject(s) - dna , cleavage (geology) , recombinant dna , chemistry , base pair , stereochemistry , biochemistry , biology , gene , fracture (geology) , paleontology
Experimental evidence indicates that the cleavage sites for site‐specific DNA cleavage enzymes are randomly distributed throughout the DNA polymer [Botchan et al. (1973) Cold Spring Harbor Symp. Quantum Biol. 38 , 383–395; Hamer and Thomas (1973) Chromosoma 49 , 243–267]. Using this information we derive equations for the probability that a DNA base pair chosen at random can be found within the population of enzymatically generated partial cleavage products whose lengths fall between L + 1 and L + r base pairs. The results describe the fraction of a long‐chain DNA that can be incorporated in a recombinant DNA partial digest library. The weight‐fraction distribution of Kuhn (1930) is a special case of the distribution derived here.