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Broadline proton magnetic resonance study of cellulose, pectin, and bean cell walls
Author(s) -
MaCkay Alex L.,
Bloom Myer,
Tepfer Mark,
Taylor Iain E. P.
Publication year - 1982
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360210805
Subject(s) - chemistry , pectin , cellulose , relaxation (psychology) , cell wall , dipole , nuclear magnetic resonance , proton , crystallography , analytical chemistry (journal) , chromatography , biochemistry , organic chemistry , physics , psychology , social psychology , quantum mechanics
We have used broadline proton magnetic resonance to study molecular motion in cellulose, a sodium pectate solution, a calcium pectate gel, and isolated bean cell walls. All samples were prepared in D 2 O to minimize the contribution of water to the observed signals. For each sample, a free induction decay was obtained, and the second moment, spin‐lattice relaxation, and dipolar relaxation were measured. Our results show that the large majority of protons in cellulose are immobile. Rigid and mobile domains were also observed in the pectate samples. We have shown that gelation induces large‐scale changes in the free induction decay, the second moment, and the relaxation behavior of the pectate. As with the other samples, rigid and more mobile domains were found in bean cell walls. The fraction in the rigid domains is much larger than the fraction of cellulose in the sample, suggesting that the noncellulosic wall components are also organized into rigid and mobile domains.