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Quasielastic light scattering by biopolymers. V. Interparticle interactions between polynucleosomes
Author(s) -
Schmitz Kenneth S.,
Parthasarathy Nambi,
Kent Jacqueline C.,
Gauntt Jennifer
Publication year - 1982
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360210707
Subject(s) - electrophoretic light scattering , chemistry , light scattering , scattering , electrophoresis , diffusion , ionic strength , quasielastic scattering , chemical physics , ionic bonding , dynamic light scattering , ion , charge density , static light scattering , range (aeronautics) , surface charge , analytical chemistry (journal) , thermodynamics , optics , chromatography , nanotechnology , small angle neutron scattering , physics , materials science , aqueous solution , neutron scattering , organic chemistry , quantum mechanics , nanoparticle , composite material
Quasielastic light scattering and electrophoretic light scattering experiments were performed on chicken erythrocyte polynucleosome solutions at various temperatures and ionic strengths. The apparent diffusion coefficient, D app , was found to depend on the scattering vector K . In general, D app can be described as a damped oscillatory function of K in the ionic strength range of 10 to 60 m M and over the temperature range of 10 to 40°C. Electrophoretic light scattering studies on total digest chromatin samples indicate the apparent charge on the polynucleosomes increases as the ionic strength is lowered from 10 to 1 m M . These data are interpreted in terms of fluctuations in the surface charge distribution of the polyion and subsequent inducement of an asymmetric distribution of small ions about the polyion. These fluctuation components lead to the formation of “clusters” of polyions.