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Flow birefringence of T7 phage DNA: Dependence on salt concentration
Author(s) -
Cairney Kim L.,
Harrington Rodney E.
Publication year - 1982
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360210506
Subject(s) - persistence length , chemistry , ionic strength , flow birefringence , ionic bonding , solvent , chemical physics , dna , bacteriophage , birefringence , polymer , thermodynamics , analytical chemistry (journal) , chromatography , optics , organic chemistry , physics , ion , biochemistry , escherichia coli , aqueous solution , gene
We have determined extinction angles and flow birefringence of T7 bacteriophage DNA over a wide range of shear, polymer concentration, and solvent ionic strength. From these data, information on the simple salt dependence of coil permeability to solvent and on short‐range intrachain interactions (persistence length) was obtained. At all ionic strengths, our results are consistent with a partially draining coil in the Gaussian subchain dynamical theory of Rouse‐Zimm‐Tschoegl‐Bloomfield. Salt dependence of persistence length is comparable to, although somewhat less than, that obtained previously using similar methods with a fivefold higher‐molecular‐weight DNA (T2 bacteriophage DNA). Possible reasons for observed discrepancies are analyzed, and the results of this work are compared in detail to other current studies of solvent ionic strength dependence in persistence length and hydrodynamic properties of DNA.