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Poly(dA‐dT) complexes with histone H1 and pancreatic ribonuclease: Specific base recognition evidenced by ultraviolet resonance Raman spectroscopy
Author(s) -
Chinsky L.,
Turpin P. Y.
Publication year - 1982
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360210204
Subject(s) - chemistry , thymine , resonance raman spectroscopy , raman spectroscopy , ribonuclease , resonance (particle physics) , random coil , rnase p , raman optical activity , bovine pancreatic ribonuclease , crystallography , excited state , spectroscopy , dna , circular dichroism , molecule , rna , biochemistry , optics , physics , organic chemistry , particle physics , nuclear physics , gene , quantum mechanics
The conformational changes of poly(dA‐dT) from random coil to ordered structure with stacked bases produce important changes in the Raman line intensities (hypochromism) when the polymer is excited under the preresonance Raman conditions (λ excitation = 300 nm). Poly(dA‐dT)–RNase and poly(dA‐dT)–histone H1 interactions have been studied as models of mechanisms of destabilization and stabilization by proteins of the DNA secondary structure, respectively, following this intense preresonance Raman hypochromism. In addition, the specific variation of the intensity of the 1582‐cm −1 line of adenine is interpreted in terms of the interaction of the amino group with the RNase (thus involving the large groove). In the poly(dA‐dT)–H1 complex, the intensity of the 1665‐cm −1 line of thymine increases. This increase appears to involve the C 2 O group of thymine, located in the narrow groove.