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Intramolecular low‐frequency vibrations in lysozyme by neutron time‐of‐flight spectroscopy
Author(s) -
Bartunik H. D.,
Jollès P.,
Berthou J.,
Dianoux A. J.
Publication year - 1982
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360210105
Subject(s) - chemistry , lysozyme , intramolecular force , neutron spectroscopy , deuterium , spectroscopy , crystallography , resolution (logic) , neutron , time of flight , spectral line , crystallite , stereochemistry , neutron scattering , atomic physics , physics , chromatography , nuclear physics , biochemistry , quantum mechanics , artificial intelligence , astronomy , computer science
High‐resolution neutron time‐of‐flight (TOF) spectra were measured for partly deuterated hen egg‐white lysozyme in solution with and without N ‐acetyl‐glucosamine inhibitor bound to the cleft, and in polycrystalline form. Weak but reproducible bands occur at frequencies between 25 and 375 cm −1 . The bands were tentatively assigned on the basis of previous results for homopolypeptides. At very small energy transfers (between about 1 cm −1 and 40 cm −1 ), the TOF spectra show a dependence both on inhibitor binding and crystalline environment. This is interpreted in terms of conformational flexibility.