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Evaluation of RNA conformation from circular dichroism and optical rotatory dispersion data
Author(s) -
Gratzer W. B.,
Richards E. G.
Publication year - 1971
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360101220
Subject(s) - optical rotatory dispersion , circular dichroism , chemistry , dispersion (optics) , consistency (knowledge bases) , pairing , rna , vibrational circular dichroism , crystallography , molecular physics , physics , optics , geometry , condensed matter physics , mathematics , biochemistry , superconductivity , gene
An analysis of optical rotatory dispersion and circular dichroism of RNA is described which leads to an estimate of the degree of base pairing. By the use of new standards for the double‐helical parts of the molecule, based on data for two‐stranded viral RNA species, a good fit between calculated and observed curves can be achieved. Where data are available the results of analyses of optical rotatory dispersion and circular dichroism in general show satisfactory consistency.