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Electro‐optical properties of nucleic acids and nucleoproteins III. Kramers‐Kronig relationships in linear birefringence and dichroism. Application to a DNA–proflavine complex
Author(s) -
Houssier Glaude,
Kuball HansG
Publication year - 1971
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360101206
Subject(s) - birefringence , proflavine , dichroism , chemistry , dichroic glass , circular dichroism , optical rotatory dispersion , linear dichroism , kramers–kronig relations , molecular physics , dispersion (optics) , optics , crystallography , dna , physics , refractive index , biochemistry
The relation between the birefringence dispersion curve in a dichroic absorption band and the corresponding dichroism spectrum is given, using the Cauchy's theorem. The theory was checked on a DNA–proflavine complex with the aid of electro‐optical measurements. An excellent agreement between the experimental results and the theoretical calculations was found. The birefringence and dichroism of the complex appear to be determined by the orientation of the macromolecule while no evidence for a contribution of the hyperpolarizabilities to the electro‐optical effect is found. The importance of the dichroism correction in the calculation of the birefringence dispersion curve is emphasized.