Premium
Biosynthèse de la cellulose bacterienne deutériée: étude par R. M. N. du taux d'incorporation et de la localisation du deutérium
Author(s) -
Barnoud Fernand,
Gagnaire Didier,
Odier Léon,
Vincendon Marc
Publication year - 1971
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360101118
Subject(s) - chemistry , deuterium , cellulose , cellulose triacetate , biosynthesis , monomer , bacterial cellulose , levulinic acid , proton nmr , nuclear chemistry , stereochemistry , organic chemistry , enzyme , catalysis , polymer , quantum mechanics , physics
The use of the NMR spectra (250 MHz) of cellulose triacetate allows the determination of the percentage of deuterium bonded to each of the six carbon atoms of the monomer residue (except for H −1 and one of the protons bonded to C 6 where the signals overlap). Deuterated derivatives of D ‐glucose and/or deuterated water were used for the biosynthesis of bacterial cellulose by Acetobacter xylinum. Analysis of NMR spectra of acetylated samples gives the following results. About 90% of the protons linked to C 1 and C 6 come from the D ‐glucose used in the nutrition medium, whereas 10% are exchanged with other sources of protons. Over 40% of the protons linked to C 2 , C 3 , C 4 , and C 5 arise from the water of the nutrition medium. Discrepancies between results of biosynthesis from deuterated water and from deuterated D ‐glucose can only be explained if more than one enzymatic process is involved in the biosynthesis of bacterial cellulose.