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Sedimentation characteristics of DNA multiply crosslinked by a difunctional alkylating agent, mustard gas
Author(s) -
Edwards P. A.,
Shooter K. V.
Publication year - 1971
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360101105
Subject(s) - chemistry , dna , dimethyl sulfoxide , sulfoxide , alkylation , denaturation (fissile materials) , sedimentation , sulfur mustard , alkali metal , degradation (telecommunications) , fraction (chemistry) , polymer chemistry , organic chemistry , biochemistry , nuclear chemistry , catalysis , paleontology , telecommunications , toxicity , sediment , biology , computer science
It is confirmed from sedimentation studies that only a small fraction of diguaninyl derivatives formed on reaction on reactions of native DNA with mustard gas crosslink the two DNA chains. It is also apparent that heavily alkylated DNA can be studied in the denatured form in dimethyl sulfoxide without the gross degradation that results if denaturation is effected by alkali.