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Polarization of fluorescence of an oriented solution of rodlike particles bearing a fluorescent label
Author(s) -
Weill G.,
Hornick C.
Publication year - 1971
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360101018
Subject(s) - polarizability , chemistry , acridine orange , fluorescence , anisotropy , fluorescence anisotropy , molecule , macromolecule , molecular physics , electric field , polarization (electrochemistry) , chemical physics , optics , physics , organic chemistry , quantum mechanics , apoptosis , biochemistry
The variation of the polarized components of fluorescence of a rodlike particle bearing a fluorescent label upon partial orientation is calculated for some special geometry of the dye macromolecules complexes. Explicit expressions are given for the case where the energy of the molecule in the field depends only on one angle θ, showing that the result is a function of both 〈sin 2 θ〉 and 〈sin 4 θ〉. For the case of orientation in an electric field through an anisotropic induced moment, the expressions allow the calculation of this anisotropy of polarizability. The method is applied to the measurement of the polarizability of rodlike fragments of DNA labeled by intercalated molecules of Acridine Orange.