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Density‐gradient sedimentation equilibrium of DNA and the effective density gradient of several salts
Author(s) -
Schmid Carl W.,
Hearst John E.
Publication year - 1971
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360101011
Subject(s) - chemistry , density gradient , sedimentation , sedimentation equilibrium , potential gradient , polyelectrolyte , thermodynamics , caesium , chemical physics , chromatography , polymer , inorganic chemistry , physics , ultracentrifuge , organic chemistry , geology , paleontology , quantum mechanics , sediment
The various treatments of sedimentation equilibrium are compared on a theoretical and an experimental basis. Particular attention is paid to the polyelectrolyte nature of the problem and the choice of a neutral component. The effective density gradients of several cesium salts for DNA are measured. Two previous theories for the effective density gradient are shown to be equivalent, and the experimental values are interpreted with respect to these theories. It is clear t hat sedimentation equilibrium in a density gradient may be used for the determination of unambiguous molecular weights.