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The circular dichroism in the ultraviolet of aminoacridines and ethidium bromide bound to DNA
Author(s) -
Dalglkish D. G.,
Peacocke A. R.,
Fey G.,
Harvey C.
Publication year - 1971
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360101008
Subject(s) - ethidium bromide , chemistry , circular dichroism , dna , ligand (biochemistry) , bromide , acridine , stereochemistry , ultraviolet , crystallography , photochemistry , biochemistry , inorganic chemistry , organic chemistry , physics , receptor , quantum mechanics
The circular dicrosim (CD) spectra of complexes of DNA with ethidiun bromnide, profiavine, 9‐aminoacridine and 4‐etliyl‐9‐amino‐acridine have been determined between 220 and 450 nm, the range lieing extended to 600 nm for ethidiufm bromide. The variation of the magnitude of the visible and near—ultraviolet CD spectra of ethidium bromide—DNA complexes with the amount of ligand bound ( r ) suggests a common binding position with profiavine. On the other hand, 4‐ethyl‐9‐aminoacndine complexed to DNA shows CD spectra not distinguishable from those of 9‐aminnoacnidmc in both the visible and ultraviolet. The interpretation of these results with respect to the stereochemistry of the DNA‐ligand complexes is discussed.