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Kinetic studies of interaction between acridine orange and DNA
Author(s) -
Sakoda Mitsuaki,
Hiromi Keitaro,
Akasaka Kazuyuki
Publication year - 1971
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360100606
Subject(s) - acridine orange , chemistry , intercalation (chemistry) , dna , base pair , photochemistry , phosphate , acridine , biophysics , biochemistry , inorganic chemistry , organic chemistry , apoptosis , biology
The interaction between acridine orange (AO) and deoxyribonucleic acid (DNA) was studied by the stopped‐flow method. The spectral change of AO due to interaction with DNA was followed over the wavelength range 350–600 nm at various concentration ratios of DNA phosphate to dye. The spectral change observed by the stopped‐flow method was found distinctly different from that, during the dead‐time, leading to a conclusion that the binding of AO to the outside of DNA occurs much faster than the intercalation into base pairs of DNA. The dependence of the rate of reaction on the reactant concentration and on the salt, concentration of the solution was also studied. The results are consistent with the mechanism that the intercalation proceeds via the outside bound state.