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Infrared spectra of transfer ribonucleic acids. III. Fragments of formyl‐methionine tRNA from Escherichia Coli
Author(s) -
Katsura Tatsuo,
Morikawa Kosuke,
Tsuboi Masamichi,
Kyogoku Yoshimasa,
Seno Takeshi,
Nishimura Susumu
Publication year - 1971
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360100407
Subject(s) - chemistry , nucleotide , transfer rna , ribonuclease , escherichia coli , fragment (logic) , stereochemistry , rna , methionine , polynucleotide , base (topology) , crystallography , amino acid , biochemistry , mathematical analysis , mathematics , computer science , gene , programming language
Four fragments (named K, L, M, and N) of Escherichia coli formylmethionine transfer RNA have been prepared by a partial digestion with ribonuclease T 1 followed by a chromatographic separation with a DEAE–Sphadex (A‐25) column and then a DEAE–cellulose column. The fragment K is the anticodon fragment with 19 nucleotides (previously reported). L is a fragment with 57 nucleotides involving the 3′‐terminal (CCA). M is a fragment with 51 nucleotides which is equal to L except that M lacks 6 nucleotides at the 3′‐terminal. N is a fragment with 20 nucleotides which involved the 5′‐terminal and corresponds to the complementary half to L. The infrared absorption spectrum has been observed of each of these fragments and two equimolar mixtures L + N and M + N in D 2 O solutions at several temperatures. The results indicate that at 37°C, K has about 4 hydrogen‐bonded base‐pairs, L about 11, and M about 13. On the other hand, fragment N is found to have only three weak G‐C pairs. For both L + N and M + N, 15–17 strong base pairs are found. The observations give direct support to the clover‐leaf structure and at the same time provide information on the stability of each of the four arms in the structure.