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Simple methods for the orientation of DNA molecules in films suitable for optical studies
Author(s) -
Kurucsev T.,
Zdysiewicz J. R.
Publication year - 1971
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360100313
Subject(s) - chemistry , acridine orange , dna , dichroic glass , molecule , dichroism , vinyl alcohol , nanotechnology , optics , polymer , organic chemistry , materials science , biochemistry , physics , apoptosis
Three methods are described for the preparation of oriented samples of DNA molecules in films. The methods involve simple equipment and produce films of good optical qualities, suitable for the determinations of dichroic spectra, for example. To illustrate the quality of the films the dichroism of DNA and of acridine orange–DNA complex are described. Evidence is given that native DNA structure is stabilized by poly‐(vinyl alcohol).