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Minor nucleosides in RNA: Optical studies of dinucleoside phosphates containing inosine
Author(s) -
Formoso Carl,
Tinoco Ignacio
Publication year - 1971
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360100309
Subject(s) - chemistry , stacking , circular dichroism , inosine , salt (chemistry) , crystallography , ionic strength , ionic bonding , stack (abstract data type) , stereochemistry , ion , biochemistry , aqueous solution , organic chemistry , enzyme , computer science , programming language
From the study of circular dichroism (CD) spectra and hypochromism we conclude that the dinucleoside phosphates IpA, ApI, and IpI stack, while IpU stacks very little. Studies with various concentrations of IpI in low salt, and 1 M NaCl indicate that the stacking geometry of this compound is sensitive to the ionic strength of solution. The CD of poly I is presented and compared to the data for IpI. Little change was found in the CD of poly (G,I) (1 : 1) with change of salt concentration, and we conclude that, unlike Poly I, there is no major structural change. From the CD of poly (G, I), IpI, and GpG, the CD of IpG plus GpI is calculated by using the nearest‐neighbor approximation. From the calculated spectrum, we tentatively conclude that there is stacking in either IpG or GpI or both.