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Fractionation of poly (dA‐dT) by gel chromatography
Author(s) -
Jang ChoongGook,
Bartl Paul
Publication year - 1971
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360100305
Subject(s) - chemistry , fractionation , chromatography , gel permeation chromatography , agarose , molecular mass , analytical chemistry (journal) , polymer , organic chemistry , enzyme
A commercial sample of poly (dA‐dT), a copolymer of 2′‐deoxyribosyladenosine (dA) and 2′‐deoxyribosylthymidine (dT) of perfectly alternating sequence, was fractionated by chromatography on Agarose gel. Paucidisperse fractions of different molecular size were obtained. The plot of log s 0 20,w values shows a linear dependence on V e . The buoyant densities of individual fractions do not differ over the molecular size range studied. On the other hand, the heat‐induced hyperchromic effect was found to depend on molecular size below a certain limit, s 0 20,w = 4.12 S.