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Interaction of proflavine and 9‐aminoacridine with DNA at temperatures below and above the melting temperature
Author(s) -
Jordan D. O.,
Sansom L. N.
Publication year - 1971
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360100214
Subject(s) - proflavine , chemistry , melting temperature , dna , dissociation (chemistry) , helix (gastropod) , biophysics , biochemistry , materials science , composite material , biology , ecology , snail
The influence of temperature on the binding of 9‐aminoacridine and of proflavine to E. coli DNA in 10 −3 M NaCl solution has been determined by a spectrophotometric technique. The inadequacy of the expression normally used for the determination of the extent of binding is discussed with reference to measurements at temperatures above which dissociation of the double helix occurs. A method of determining the relative extents of binding to native and denatured DNA at elevated temperatures is described.