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Interactions of metal ions with polynucleotides and related compounds. III. Degradation of polyribonucleotides by lanthanum ions ,
Author(s) -
Eichhorn Gunther L.,
Butzow James J.
Publication year - 1965
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360030109
Subject(s) - polynucleotide , chemistry , lanthanum , nucleotide , metal ions in aqueous solution , nucleoside , metal , phosphate , inorganic chemistry , ion , chelation , oligonucleotide , cleavage (geology) , stereochemistry , organic chemistry , biochemistry , dna , geotechnical engineering , fracture (geology) , gene , engineering
Abstract The polyribonucleotides of adenylic, cytidylic, and uridylic acids are degraded in a 2‐hr. period at 64°C. into low molecular weight oligonucleotides and mononucleotides through the action of lanthanum (III) or other trivalent inner transition metal ions. Polyinosinic acid is degraded somewhat more slowly. Cleavage of the polynucleotide chains occurs at the 5′‐phosphate linkages. The 3′‐mononucleotides produced require over 100 hr. of further heating at 64°C. to produce nucleoside and orthophosphate. The reaction between lanthanum (III) and the polynucleoside involves one metal ion for every three nucleotide residues, with each metal ion apparently chelated to a 2′‐hydroxyl and a phosphate group and bound to only one polynucleotide chain.