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Crosslinked synthetic polypeptides. I. Preparation and characterization of crosslinked poly Glu 51 ‐Lys 33 Tyr 16 and of poly Glu 52 Lys 33 Tyr 15 no. 3
Author(s) -
Marfey Peter S.,
Gill Thomas J.,
Kunz Heinz W.
Publication year - 1965
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.360030105
Subject(s) - chemistry , hydrolysis , molecule , sephadex , monomer , amide , solubility , deamination , stereochemistry , polymer chemistry , chromatography , organic chemistry , polymer , enzyme
Crosslinked synthetic polypeptides of poly Glu 51 Lys 33 Tyr 16 (mol. wt. 31,000) and of poly Glu 52 Lys 33 Tyr 15 (No. 3) (mol. wt. 52,000) containing from one to six crosslinks per molecule have been prepared by use of FFDNB, WRK and ICD reagents. The monomeric fractions of these derivatives were isolated by Sephadex G‐100 chromatography. The number of crosslinks per molecule of DNPene derivatives was determined by total hydrolysis of the derivatives, isolation of O , N ∈ ‐DNPene‐Tyr‐Lys bridges by paper chromatography and then spectrophotometric quantitation. The number of the amide‐type crosslinks in Am derivatives was established by their deamination followed by total hydrolysis and quantitation of the remaining lysine residues. Crosslinked derivatives appear to have a more compact structure, as judged by their behavior on the Sephadex columns and by their intrinsic viscosities. They were further characterized in regard to their amino acid composition, average number of the crosslinks per molecule, nitrogen content, solubility, root‐mean‐square end‐to‐end distance, and their spectral properties. Their properties recommend them as useful models for the study of the tertiary structure of proteins in solution.