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Cognate base‐pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase
Author(s) -
Kimoto Michiko,
Soh Si Hui Gabriella,
Tan Hui Pen,
Okamoto Itaru,
Hirao Ichiro
Publication year - 2021
Publication title -
biopolymers
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.556
H-Index - 125
eISSN - 1097-0282
pISSN - 0006-3525
DOI - 10.1002/bip.23407
Subject(s) - chemistry , dna ligase , base pair , dna , ligation , stereochemistry , biochemistry , microbiology and biotechnology , biology
We present cognate base pair selectivity in template‐dependent ligation by T4 DNA ligase using a hydrophobic unnatural base pair (UBP), Ds‐Pa. T4 DNA ligase efficiently recognizes the Ds‐Pa pairing at the conjugation position, and Ds excludes the noncognate pairings with the natural bases. Our results indicate that the hydrophobic base pairing is allowed in enzymatic ligation with higher cognate base‐pair selectivity, relative to the hydrogen‐bond interactions between pairing bases. The efficient ligation using Ds‐Pa can be employed in recombinant DNA technology using genetic alphabet expansion, toward the creation of semi‐synthetic organisms containing UBPs.